Alternaria blight is the most common and devastating disease caused by Alternaria cucumerina var. cyamopsidis in Clusterbean. This fungus grows well in Potato dextrose agar and Tomato dextrose agar medium under in vitro conditions. The growth of the fungus was tested using fifteen different media. PDA appeared to be better than other media for the growth of Alternaria cucumerina var. cyamopsidis. The growth characteristics of the fungus such as colour of the colony and sporulation were also different in different culture media. The colony of the fungus was dark brown in colour. Maximum sporulation of the test fungus was found on PDA medium whereas no sporulation was seen on Czapeck’s dox agar medium. Thus the present work will be useful for further investigation on the physiology of the fungus and management of the disease. | Assessment of different culture media on the growth and sporulation of Alternaria cucumerina var. cyamopsidis causing alternaria blight of clusterbean