During the present study, mitotic chromosomes of freshwater snail Sphaerium indicum were studied using gills. The observed diploid chromosome number was 2n=30 consisting of 26 metacentric pairs and 04 submetacentric pairs respectively. Chromosomes were studied using Giemsa staining and two banding techniques viz. C-banding and Ag-NOR banding. Preliminary C-band studies revealed the presence of terminal blocks besides the presence of centromeric heterochromatin. The nucleolar organizer region (NOR) was found on one chromosome pair . on the submetacentric chromosome pair no. 5. The cytogenetic relationship between S. indicum and other species of Veneroida was also discussed. | Chromosomal and banding studies in Sphaerium indicum Bivalvia Mollusca