The experiment was undertaken to assess the magnitude of combining ability for yield, its components and fibre properties in upland cotton. The experimental material . 52 genotypes (15 parents, 36 crosses and 1 check) produced from line x tester mating design were evaluated for fifteen characters in randomized block design with three replications across 3 environments at viz., Cotton Research Unit, Dr. PDKV, Akola, Regional Research Centre, Amravati and ICAR-Central Institute for Cotton Research, Nagpur during kharif, 2017-18. | Combining ability studies for yield its component traits and fibre properties through line X tester mating design in upland cotton Gossypium hirsutum L.