Otitis media is a major health problem and occurs with high incidence and prevalence in both developed and developing countries. This study was undertaken to determine etiological agents of the otitis media and their antimicrobial susceptibility patterns. Ear swabs collected from clinically diagnosed otitis media were subjected to microbiological investigations. Their antibiogram was determined by Kirby-Bauer disc diffusion method. Out of 248 ear swabs processed 198 () samples showed growth of various bacteria. 113 () gram positive and 99 () gram negative bacteria were isolated. This study shows the rate of isolation of various organisms from the ear swabs and their tendency towards antibiotic resistance. | Bacteriological study of patients with otitis media attending tertiary care hospital