The present study aimed at evaluating the BCG vaccine immunogenicity testing in young sahiwal calves. The bTB screening and vaccine immunogenicity were evaluated using the OIE approved Interferon gamma release assay (IGRA) using BOVIGAM™ kit. BCG was titrated with 105 colony forming units (CFU) and observed from the present study that low dose of 105 (CFU) is found to be effective for inducing significant bTB specific protective IFN-gamma at day 14 post vaccination. Thus the conventional BCG vaccination shall be considered as an approach for the control as well as reducing the bio burden of bovine tuberculosis along with complementary diagnosis. | Evaluation of bacillus - calmette- guerin BCG immunogenicity in indigenous calves under field condition
