Bovine herpesvirus-1 (BHV-1) a member of the Herpes viridae family and Alphaherpes viridae subfamily genus Varicello virus is a most common viral pathogen found in bovine semen. The entry process of Alphaherpes viruses consist of two steps, initial virus attachment and subsequent virus penetration involving membrane fusion as in other alphaherpes viruses, BHV-1 glycoproteins are the major structural components of the viral envelope and virus-infected cell membranes. Glycoprotein D(gD), of BHV-1 is one of four major glycoproteins, namely gB, gC, gD, and gH that have been identified on the virus envelope and the plasma membranes of BHV-1-infected cells [14, 24]. Total of 73 open reading frames (ORFs) have been clearly identified which are homologous to genes found in other alpha herpes viruses. | Expression profiling of glycoprotein d gene of bovine herpes virus in madindar by bovine kidney cells via real time PCR