Detection of viral antigen by direct fluorescent antibody test (FAT) from clinical samples collected from cattle and buffaloes of Navsari, Surat and Valsad Districts of Southern Gujarat were carried out. Out of a total of 116 clinical samples (44-cattle, 72-buffaloes) tested, fluorescence was observed in 14 () samples. Species-wise, eight cattle () and 6 buffaloes () were found to be positive by the test. The highest number of animals were detected from Surat () followed by Valsad () and least in Navsari () district. Viral antigen detected in (4/50), (1/14), (2/7) in cattle and , (2/12), (1/6), (3/26) in buffaloes from nasal, conjunctival and vaginal swabs, respectively. Only placental cotyledon sample collected from cattle showed positive reaction by direct FAT. | Detection of bovine Herpesvirus-1 infection in bovine clinical samples by direct fluorescent antibody test
