This study was designed to isolate and determine the antibiotic sensitivity pattern of bacteria associated with the hands of commuters in Akure metropolis. The samples were collected using sterile swab sticks at different motor parks (Obaile, Oke-Ijebu, Benin, FUTA and Road block), then transported to Microbiology laboratory for further analyses. Isolation of microorganisms was carried by direct plating on the bacteriological media. The plates were incubated at 37oC for 24 hours. Enumeration of the bacterial isolates was done by direct count of the colonies formed on the plates. Pure bacterial isolates was obtained by repeated streaking on the fresh media. | Isolation enumeration and antibiotic sensitivity pattern of bacteria from the hands of commuters in Akure metropolis