Root and collar rot of okra incited by Macrophomina phaseolina is an emerging problem in many parts of Gujarat. Twenty okra genotypes were screened for their resistance to root and collar rot under field conditions for two seasons. For further confirmation of the field results, variability in protein profile of resistant and susceptible genotypes was studied using SDS PAGE. Results revealed that protein profile of genotypes exhibited considerable variation from each other. Number of bands varied from four in highly susceptible variety to nine in resistant variety. Moderately resistant and susceptible genotypes had seven and six bands, respectively. The four genotypes were grouped into two clusters viz., cluster A (Resistant and moderately resistant) and cluster B which had two sub clusters (B1- Susceptible and B2- Highly susceptible). Similarity coefficient ranged from – . | Protein profiling of okra genotypes resistant to root and collar rot incited by Macrophomina phaseolina Tassi goid. using SDS-PAGE