For many crucial experiments in gene cloning and further identification, purified and high quality genomic DNA is the prerequisite. In plants, polysaccharides and secondary metabolites impurities removal is complicated and time-consuming protocols are available for pigeon pea plant. In present study, rapid and proficient method for DNA extraction was optimized. The method utilizes small amount of plant material to lessen inhibitory agents like polysaccharides and phenolic compound. The method involved crushing of the young leaf in lysis buffer, incubation at 65ºC, extraction by phenol: chloroform: isoamyl alcohol and finally DNA precipitation by cold ethanol. The results showed highly pure DNA. | Rapid procedure for genomic DNA extraction from pigeon pea
