A study was carried to observe the changes in the Haematological parameters such as Total Erythrocyte count (TEC), Total Leukocyte Count (TLC), Haemoglobin (Hb), Packed Cell Volume (PCV), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), Total Platelet count and Differential Leukocytes count (DLC) of crossbred cows Karan Fries (KF) (Holstein Fresian × Kankrej) cows during transition period. Blood samples were collected from the jugular vein of the selected animals on -30, -15, -7, 0, +7, +15, + 30 days with respect to expected date of calving. TEC and Hb non-significantly increased on the day of calving. A | Study of haematological parameters of crossbred cows during peripartum period