A set of 18 F1 hybrids developed as a result of line x tester mating design involving six lines and three testers were evaluated in randomized block design in three replications for heterosis in four quality traits. Heterosis to the extent of , , and over mid parent and , , and over better parent was recorded for number of locules per fruit, pericarp thickness, total soluble solids and ascorbic acid content, respectively. Standard heterosis over Lakshmi to the extent of , , and was recorded for number of locules per fruit, pericarp thickness, total soluble solids and ascorbic acid content respectively and over US-618 to the extent of , , and was recorded for number of locules per fruit, pericarp thickness, total soluble solids and ascorbic acid content respectively. The crosses LE-67 × Pusa Gaurav, LE-65 × Pant T-3, LE-62 × Pant T-3 and LE-64 × Pant T-3 for number of locules per fruit, pericarp thickness, total soluble solids and ascorbic acid content were identified as a best heterotic combination, respectively. | Heterosis for quality traits in tomato Solanum lycopersicum L.