The present study was done with the objective of finding the differential expression of ISG 15 and MX 2 genes in pregnant and non pregnant heifer and pleuriparous buffaloes. A total of 40 heifer and pleuriparous buffaloes were selected and divided into 2 experiments, Experiment I consisted of heifers (Group I and Group II) and Experiment II (Group III and Group IV) consisted of pleuiparous buffaloes. | Interferon stimulated gene 15 and myxovirus resistance 2 genes are upregulated during early pregnancy in buffaloes