Pseudomonas aeruginosa is a classical opportunistic pathogen with innate resistance to many antibiotics and disinfectants. Clinical samples which yielded Pseudomonas aeruginosa were included in this study. Antibiogram of the isolates was determined by Kirby-Bauer disc diffusion method. A total of 339 pseudomonas aeruginosa were isolated from various clinical samples during the period of study. Majority of the isolates in our study were obtained from Pus / Wound swabs followed by other samples. Resistance to amoxicillin +Clavulinic acid () was high in our study followed by resistance to ciprofloxacin, ceftazidime and amikacin. This hospital data will help in formulating antibiotic polices and in implementation of better infection control strategies. Periodic antimicrobial surveillance to monitor the resistance patterns of these organisms is thus required. | Isolation and antimicrobial susceptibility pattern of pseudomonas aeruginosa from various clinical samples at a Tertiary care hospital