A set of 45 polymorphic microsatellite markers, representing markers for almost each chromosome, was used for the assessment of genetic diversity in 35 genotypes of bread wheat. A total of 127 alleles were detected which ranged from 2 to 6 with an average allele number of per locus. The PIC value of the 45 SSRs ranged from to with an average PIC of . Resolving power and Marker index of all 45 markers varied from to and to , respectively. | Microsatellite based genetic divergence study in bread wheat Triticum aestivum L. em. Thell.