The present investigation was carried out at ZAHRS, UAHS, Shivamogga during 2016- 2017 Kharif season to study the genetic variability, heritability and genetic advance, in F3 generation of FCV (Flue Cured Virginia) tobacco cross, TB-70 x TB-102. The mean and variance in sixteen quantitative characters in F3 generation of the cross TB-70 x TB-102 indicated wide variability among the families for all the traits viz., leaf area per plant, internodal length, plant height, chlorophyll content, specific leaf weight, number of leaves, leaf length, leaf breadth, green leaf yield, cured leaf yield, top grade equivalent, reducing sugar content and nicotine content. GCV and PCV values were relatively higher for leaf area per plant. | Assessment of genetic variation in F3 generation of FCV tobacco Nicotiana tabacum L.