This study aimed to determine enteroviruses using different PCR methods and also studying the effect of ethanolic extract of Nigella Sativa on isolated viruses in vitro. we performed reverse transcription-PCR (RT-PCR), nested PCR and cell culture-PCR followed by real time PCR to monitor the occurrence of enteric viruses in in 23 samples collected from irrigation water sites in El-Giza and El-Menofya Governorates from March 2017 to May 2017; fourteen samples from five rural sites in El-Menofya which no sanitation systems service these regions and nine samples from three urban sites in El-Giza served by sanitation systems were investigated. | Characterization of enteroviruses in irrigating water in Egypt and in clinical samples with the assessment of ethanolic extract of nigella sativa in response to these viruses
