The plant Acacia catechu () Willd hydroethanolic leaf extract was subjected to qualitative and quantitative phytochemical screening, HPTLC and GCMS analysis. The phytochemical analysis revealed the presence of carbohydrates, proteins, flavonoids, total phenolics, glycosides, total free amino acids, tannins, alkaloids and thiols. The secondary metabolites like flavonoids, tannins and total phenolics were quantified as 82μg/ml, and . | Phytochemical screening HPTLC and GCMS profile of Acacia catechu Willd Hydroethanolic leaf extract