This study was conducted on one hundred and fifty clinical isolates of S. aureus isolates forming three groups (group one fifty community acquired isolates from adult infections, group two: fifty community acquired isolates from pediatric infections and group three:fifty hospital acquired isolates as a control group) isolated from different clinical specimens. All isolates were identified by conventional methods such as morphological identification, gram stain, and catalase test. Identification to species level by slide and tube coagulase, culture on DNase agar and mannitol salt agar | Rapid detection of panton-valentine leukocidin PVL gene from staphylococcus aureus clinical isolates
