The 16S rRNA gene is composed of highly conserved, specie-specific sequences between different species of bacteria and the application of restriction endonucleases on the amplified 16S rRNA gene is a novel diagnostic tool in molecular characterization of bacterial isolates. In a study aimed at characterizing bacteria using some selected endonucleases, a total of 31 isolates collected from the stock culture in the Department of Medical Laboratory Science were re-identified using standard microbiological procedures as Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Alcaligenes faecalis. The isolates were subjected to antibiotic susceptibility testing to ascertain their susceptibility pattern using the agar disc diffusion method; followed by DNA extraction, purification, amplification, gel electrophoresis and sequencing. | Restriction sites annotation on Klebsiella 16s ribosomal RNA using selected sticky end cutting endonucleases
