To isolate the bacterial pathogens causing asymptomatic bacteriuria in antenatal women,to study the drug resistance pattern and prevalence of MRSA and ESBL producers. Bacterial pathogens were isolated from 1000 urine samples collected from antenatal women following standard procedures. All the isolates were studied for antibiotic susceptibility pattern using modified Kirby bauer disc diffusion method. Resistant isolates were identified and studied for MRSA and ESBL by various methods. Out of 1000 urine samples culture positive were 118(). was the predominant organism isolated 54 ( %).All Gram negative bacilli were 100% sensitive to Piperacillin –tazobactam and Imipenem. All Gram positive cocci were 100% sensitive to Vancomycin and Linezolid. No MRSA was detected. Among the Enterobacteriaceae isolates, 6(8%) were found to be ESBL producers. As asymptomatic bacteriuria is related to the complications in pregnancy, it is imperative that Urine culture tests should be done routinely for all the pregnant women to find out asymptomatic bacteriuria, and every positive case should be managed with appropriate antibiotics, to prevent any complication related to pregnancy. | Isolation of bacterial pathogens and detection of MRSA and ESBL producers causing asymptomatic bacteriuria in antenatal women