Hand hygiene is considered as the most effective method of controlling health care related infections. The present study was conducted to determine the awareness of hand washing among Health care personnel (HCP) and to compare the microbial flora of dominant and non-dominant hands of HCP. Sixty-nine HCP were interviewed using a pre-validated questionnaire regarding compliance of hand washing practices. Their dominant hand was noted. Using the sterile pre-moistened swab sticks sample was collected from both hands of HCP and cultivated on Blood Agar. | Cross-sectional study of hand hygiene practices and comparison of microbial flora of dominant and non-dominant hand amongst health care personnel