The present investigation was undertaken for the isolation and characterization of B. thuringiensis strains from different habitats based on the morphology, staining properties and biochemical characters. Fifty four samples including soil samples (30), phylloplane (21), insect cadaver (2) and stored grain dusts (1) were collected from cultivated and barren lands not having Bt history. | Isolation and characterization of native isolates of Bacillus thuringiensis from Vidarbha region