The present study was carried out to isolate Methicillin-Resistant Staphylococcus aureus (MRSA) in clinical samples. Standard microbiological and biochemical methods were used to screen 404 clinical specimens comprising of pus, urine and isolates were MRSA out of 194 Staphylococcus aureus isolates. MRSA is increasing. Most MRSA are resistant to several non-beta lactam antibiotics. Frequent monitoring of susceptibility patterns of MRSA and formulation of a definite antibiotic policy may be helpful in decreasing incidence of MRSA infection. | Prevalence of MRSA among clinical isolates of staphylococcus aureus and its antibiotic susceptibility pattern at a tertiary care hospital