The survivability of the dietary culture in the presence of acid and bile salts during the passage through the gastro-intestinal tract is the pre-requisite for probiotics to function in the intestine. While the spore-forming Bacillus strains have created enormous interest as probiotics in human as well as livestock animals in the recent years, intrinsic functional properties for screening probiotics such as resistance to acid, tolerance to bile salt, cell surface hydrophobicity and rate of acid production were studied with 47 spore-forming Bacillus isolates collected from milk, soil and tomato sources. | Functional properties of spore-forming bacillus strains Pre-requisite for probiotic functions