Genetic diversity study involving twenty one thermo tolerant tomato genotypes was carried out for twelve physiological and biochemical characters at Indian Agricultural Research Institute, New Delhi during summer season of the year 2014. All the genotypes were grouped into 4 distinct clusters using D2 statistics. Cluster I and cluster II comprised of 10 genotypes represented by heat tolerant genotypes while cluster III showed 4 heat sensitive genotypes. The clustering analysis of all genotypes showed that heat sensitive and heat tolerant genotypes were represented by distinct clusters. Maximum intra cluster distance was recorded in cluster IV (), while the inter cluster distance was found maximum between cluster III and cluster IV (). Cluster means of all genotypes showed that genotypes in cluster I exhibited minimum cluster mean value for chlorophyll b ( mg/100g) and maximum for proline content (364µg/g). | Genetic diversity studies in thermo tolerant genotypes of tomato Solanum lycopersicum L.