The nosocomial infections are most important medical complications in all areas of the world and they remain one of the main sources of morbidity and mortality in hospitalized patients. Acinetobacter bacteria has appeared over the last era as significant opportunistic pathogen liable for nosocomial infections, although it is commonly related with benign colonization of hospitalized patients. The present investigation is carried out to isolate, identify Acinetobacter species from the different samples collected from tertiary care hospitals. Further susceptibility and resistance of different antibiotics against Acinetobacter is performed in the present study. | Isolation characterization and antibacterial susceptibility test of Acinetobacter species obtained from tertiary care hospital