The CuO-N/ITO electrode showed the best electrochemical properties for glucose detection in comparison to the others. Chronnoamperometry of CuO-N/ITO electrode to the glucose response showed excellent stability, the linear range of 1 µM to 3600 µM with high sensitivity of µAcm−2mM−1 and µM of the detection limit (S/N = 3). A good response of the CuO-N/ITO electrode, which was investigated for different human serum samples, indicates a high potential of its towards a glucose sensor for analysis in real examples. | Facile synthesis of CuO ITO film via the chronoamperometric electrodeposition for nonenzymatic glucose sensing