Paphiopedilum orchids, the most popular and rare orchid genera, are used as potted flowers and cut flowers. In nature condition, the orchid population is threatened with extinction due to over-exploitation and altered habitat. Reduction in this orchids via large-scale micropropagation is a better option to reduce the wild harvest, meets the commercial needs as well as conserves this threatened orchid. This orchid can be propagated by germinating seeds under in vitro culture condition; however, they are difficult for micropropagation, especially regenerating plantlets from in vitro culture. This topical review aims to provide some techniques on Paphiopedilum spp. micropropagation as well as improve the culture process from ex vitroderived explants of mature plants. | Some methods in micropropagation and breeding of Paphiopedilum spp.
