In the current study we analysed the effect of mercuric chloride (HgCl2) on surface sterilization of the explants at different durations of time (1-5mins). It was observed that, longer the duration of treatment, lower was the bacterial contamination (8% contamination at 5 min duration of HgCl2 treatment) but the survival percentage of the culture decreased as exposure time increased (35% at 5 min). 3 min of exposure gave the best result for both bacterial contamination (18%) and survival percentage (68%) among other time durations. Fungal contamination doesn’t showed any significant difference at different time intervals. |
