Biofilms have the ability to attach to both biotic and abiotic surfaces. With the advent in medical industry, use of indwelling prosthetic devices have increased abiotic surfaces providing opportunities for attachment by the biofilm forming bacteria and thus leading to infections. Treatment of infection by biofilm forming bacteria is difficult due to their decreased susceptibility to antibiotics. Hence, this study was aimed to compare the sensitivity and specificity of the three phenotypic methods for the detection of biofilms. | 2020 9 7 1094-1099 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 7 2020 Journal homepage http Original Research Article https Comparative Evaluation of Different Phenotypic Methods for Biofilm Formation among Uropathogenic E. coli UPEC Rashmi P Mahale1 M. N. Sumana2 K. Anuradha3 and Adline Princy4 1 Dept. of Microbiology JSS Medical College amp Hospital JSSAHER Mysore India 2 Department of Microbiology MMC Mysore India 3 Department of Biotechnology Sastra Deemed University Thanjavur Tamil Nadu Corresponding author ABSTRACT Keywords Biofilms have the ability to attach to both biotic and abiotic surfaces. With Biofilm Infections the advent in medical industry use of indwelling prosthetic devices have Decreased susceptibility increased abiotic surfaces providing opportunities for attachment by the Phenotypic methods biofilm forming bacteria and thus leading to infections. Treatment of Article Info infection by biofilm forming bacteria is difficult due to their decreased susceptibility to antibiotics. Hence this study was aimed to compare the Accepted sensitivity and specificity of the three phenotypic methods for the detection 11 June 2020 Available Online of biofilms. 10 July 2020 Introduction substances EPS .3According to National Institute of health NIH 65 of microbial Bacteria exist in two growth modes in nature infections and 80 of the chronic infections one as the planktonic form and the other as are due to biofilm forming microorganisms. sessile aggregates called biofilm. Existence in Biofilms have the ability to attach to both aggregates as Biofilm confers the bacterial biotic and abiotic surfaces. With the advent in community certain survival advantage like medical industry use of indwelling prosthetic evading the host immune responses devices have increased abiotic surfaces resistance to antibiotics biofilm also entraps .