Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. The aim of this study was to set up a protocol for large scale for in vitro propagation of Hydrangea macrophylla, for this purpose two type of explant (shoot tip and node) were sterilized and the best percentage of survival explants (40%) was obtained when explants immersed for 20 min in or % NaOCl. For in vitro multiplication, Murashige and Skoog medium supplemented with mg/l BA and mg/l Kin was the best treatment on shoot number. Using GA3 at mg/l was a marked increase in plant length when compared with zero level (control). |