Nowadays selection and validation of endogenous gene is commonly being studied worldwide for differential gene study. And it is also considered as inevitable for gene validation studies. Endogenous gene expression varies profoundly with every cell and tissue with even mere deviations at nominal levels too, thus a need of normalization. So far, the most refined and steadfast method is preferably Quantitative Real-Time PCR (qRT-PCR) for precise quantification of gene expression to understand explicit gene functions at various development phases and beneath diverse environmental conditions that plant encounters during its life. |
