Therefore in this study immunodominant region in TP53 gene was amplified and cloned in prokaryotic expression vector. Further the conditions were optimized for the expression of the recombinant protein. The recombinant protein was purified using Ni-NTA affinity chromatography. The hyperimmune sera were raised in rabbits against the recombinant protein. In the hyperimmune sera, antibodies specific to TP53 were detected by Indirect ELISA, confirming the serological reactivity of the immunodominant region. |
