The study was undertaken to isolate the DNA of good quality from the banana wilt pathogen Fusarium oxysporum f. sp. cubense (Foc) directly from infected tissues for molecular studies. In this method, laborious and time-consuming procedures such as isolation of the pathogen and its artificial cultivation are avoided. Moreover, grinding of the fungal mycelium using liquid nitrogen is also not needed as the DNA is directly extracting from infected banana tissues. |