For this study, disease infected leaf samples were collected from pulse pathology block situated at Normon E. Borlaug Crop Research Centre, Pantnagar based on cultural, morphological and disease potential studies and then evaluated the pathogen against five botanicals viz. Calotropis, Ocimum, Aloevera, Neem and Bael at 5, 15, 25 and 35 per cent concentration through poison food technique and two bioagents viz. | 2020 9 6 2627-2636 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 6 2020 Journal homepage http Original Research Article https Potential of Botanicals and Biocontrol Agents against Rhizoctonia solani Kühn Incitant of Web Blight Disease of Mung Bean An invitro Evaluation Sadhna Chauhan L. B. Yadav K. P. S. Kushwaha and Manoj Kumar Chitara Department of Plant Pathology College of Agriculture GBPU A amp T Pantnagar Uttrakhand 263145 India Corresponding author ABSTRACT The antagonistic activity of biocontrol agents and botanicals was evaluated against Rhizoctonia Keywords solani Kühn which is reputed as one of the destructive plant pathogens and a ubiquitous soil fungus. The pathogen causes huge losses in the production of mungbean in India. For this study disease Botanicals infected leaf samples were collected from pulse pathology block situated at Normon E. Borlaug Crop bioagents web Research Centre Pantnagar based on cultural morphological and disease potential studies and then blight Rhizoctonia evaluated the pathogen against five botanicals viz. Calotropis Ocimum Aloevera Neem and Bael at solani Trichoderma 5 15 25 and 35 per cent concentration through poison food technique and two bioagents viz. harzianum and Trichoderma harzianum Th-14 and Pseudomonas fluorescens Psf 173 by dual culture technique Pseudomonas in the laboratory of the Department of Plant Pathology College of Agriculture GBPUA amp T fluorescens Pantnagar in 2017. Observations were taken after the full growth of the control in case of botanicals and after 24 48 and 72 hours in case of biocontrol agents for the assessment of the inhibitory effects. Article Info In the presence of botanicals the variability in the growth inhibition over control was observed as the maximum growth inhibition was observed in Calotropis @ 5 15 25 and 35 concentration 71 Accepted 81 84 and 86
