Three bio-agents . Trichoderma viridae, Trichoderma harzianum and Trichoderma aspergillum were evaluated against Botrytis cinerea under in vitro condition. Culture disc of fungal bio-agents and pathogen were taken from the growing cultures and transferred to PDA contained in 70 mm petridishes on opposite sides approximately at one cm from the wall of the plate. Among these three bio-gents Trichodema harzianum showed best mycelial growth inhibition of Botrytis cinerea. | 2020 9 6 1785-1789 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 6 2020 Journal homepage http Original Research Article https Invitro Efficacy of Bio-Agents against Gray Mold of Orange caused by Botrytis cinerea Sania Pallabi Reang Department of Plant Pathology School of Agricultural Uttaranchal University Dehradun-248007 Uttarakhand India Corresponding author ABSTRACT Keywords Three bio-agents . Trichoderma viridae Trichoderma harzianum and Bio-agents Botrytis cinerea Gray mold Trichoderma aspergillum were evaluated against Botrytis cinerea under in Orange vitro condition. Culture disc of fungal bio-agents and pathogen were taken from the growing cultures and transferred to PDA contained in 70 mm Article Info petridishes on opposite sides approximately at one cm from the wall of the Accepted plate. Among these three bio-gents Trichodema harzianum showed best 18 May 2020 Available Online mycelial growth inhibition of Botrytis cinerea. 10 June 2020 Introduction The origins of most citrus fruits are to be the Southern slope of Himalayan region the Citrus is the collective term containing a entire north-eastern region of India and number of species and varieties of fruits adjacent China. Oranges have their origin in known to world over for their characteristic China. flavor and attractive range of colours. The orange Citrus sinensis is the fruit of citrus The major producers of oranges are Mexico species. Ctrus sinensis is also called as sweet USA India and Argentina. Oranges are orange which is differentiate it from the bitter extensively grown in Nagpur regions of orange Citrus aurantium . The juice of Maharashtra Coorg of Karnataka Darjeeling orange includes about 12 percent sugars of West Bengal Sikkim North-Eastern region percent titrable acidity and other soluble of India Himachal Pradesh and Punjab. solids 1 percent .
