The metabolic activities of the casein peptides were verified using purified enzymatic inhibition studies. The regulatory enzyme inhibition led us to conclude a positive correlation with the role of casein peptides in modulating the diabetic and hypertensive metabolic pathways in the biological system. Purified enzymes were used for the investigation to clearly observe the direct impact on the diabetes and hypertension related bioprocesses. In the current study, the effect of chymotrypsin treatment was also investigated on the casein hydrolysates and their effects on regulatory metabolic enzymes were further analyzed. | 2020 9 6 1246-1255 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 6 2020 Journal homepage http Original Research Article https Antidiabetic and Antihypertensive Properties of Chymotrypsin Treated Cow Milk Casein Santosh Kumar1 SumanKumari2 Vipan Kumar3 and Deepak Kumar4 1 MDETT Research Center BAIF Development Research Foundation Dharoli Jind 126113 Haryana India 2 Dolphin PG Institute of Biomedical amp Natural Sciences Dehradun Uttarakhand India 3 Department of Veterinary Microbiology Khalsa College of Veterinary and Animal Science Amritsar Punjab India 4 Department of Pharmaceutical Chemistry Dolphin PG Institute of Biomedical amp Natural Sciences Dehradun Uttarakhand India Corresponding author ABSTRACT Milk is known to contain a number of peptides fractions that affect metabolic responses. Keywords Casein being the most abundant milk protein after enzymatic treatment generates peptide fragments which have unique ability to regulate the diabetic and hypertensive metabolic Angiotensin pathways in the biological system. Upon oral administration of bioactive peptides may converting affect the major body system-namely the cardiovascular digestive immune and nervous enzyme ACE systems showing multiple biogenic effects such as antimicrobial Immuno modulatory Amylase Antihypertensive antioxidative antithromboic antidiabetic and antihypertensive. In the present study the Imunomodulation peptide formulation derived from chymotrypsin treated cow milk casein was functionally Biogenic peptides studied for their antidiabetic via α-amylase inhibition and antihypertensive via angiotensin converting enzyme inhibition activity. The peptide content and their biogenic Article Info effects varies with the duration of enzymatic treatment thus depicting variable effects. Maximum antidiabetic effect was shown by bioactive derived after 3 hours of .
