Experimental conditions were established which yielded a 10-fold dynamic range (DR) for detection of MCT1 over MCT4 (antibody concentration μg/mL; integration time seconds) and a 5-fold DR of MCT4 over MCT 1 (8 μg/100 μL and seconds). The IF method was sufficiently sensitive to detect both MCT1 and MCT4 in CTCs harvested from cancer patients. |