A flow cytometric protocol amenable to the crop was standardized by comparing four buffer compositions, three sample sizes and four incubation periods for isolated nuclei using Pisium sativum cv. Citrad (2C=) as an internal reference standard. The study revealed significant differences for 2C DNA content among the accessions ranging from to , with difference. The mean 2C DNA content of tall accessions and in dwarfs, corresponding to 1C genome size of Mb and , respectively. |
