Trichoderma species is one of the key potential bio-control agents against soil-borne pathogens. In this study molecular and biochemical characterization were done using twenty four potential isolates of Trichoderma species, based on internal transcribed spacer (ITS 1 & 4), translation elongation factor(tef-1) gene region and hydrolytic enzymes. In this studytef-1 was found to be better than ITS, to distinguish the Trichoderma isolates into two different species viz., Trichoderma virens and Trichoderma harzianum, on the basis of maximum parsimony sequence analysis. |
