Infectious Bursal Disease (IBD) is an economically important and contagious viral disease of poultry. An attempt was made for development of a synthetic peptide based diagnostic reagent. Cell culture adapted viral antigen was prepared by successful passage on CEF and then ultracentrifugation to get partially purified virus. Confirmation of the virus in was done by RT- PCR. The antigen was then used for the production of hyper immune sera in rabbits. Sera samples were also collected from known IBD vaccinated and non-vaccinated birds. Four different sequences were selected based on antigenic determinant analysis using sequence data available on Gene bank. Four MAPs, M1, M2, M3 and M4 were synthesized based on these sequences using FMOC chemistry. |
