This study we attempt to (i) discover a better performing electrode material to minimize the internal resistance of the MFC, (ii) modify the anodic electrode spacing to determine a design that better supports the electrochemical activity of acting bacteria, and (iii) reduce losses during the electron transfer process (from the electron donor at the anode to the electron acceptor at the cathode) via optimization of the cathode oxygen supply rate and the external resistance using polarization analysis. |