In the present study, we use LPS to treat C2C12 skeletal muscle cells and observe expression of several inflammatory makers. Our results show that expression of inflammatory cytokine IL6 mRNA is strongly induced in the LPS-treated C2C12 cells compared to the control cells. Surprisingly, expression of the upper controller of inflammation TLR2 mRNA in the LPS-treated C2C12 cells is similar as that in the control cells. | EXPRESSION OF IL6 mRNA IS INDEPENDENT WITH EXPRESSION OF TLRS MRNA IN LIPOPOLYSACCHARIDE TREATED MYOTUBES Abstract. Obesity related metabolic disorders such as insulin resistance and type 2 diabetes are raising as critical health problems of modern world. Obesity induces increased plasma level of lipopolysaccharide LPS that contributing to system chronic inflammation. This response has been shown as a marked factor linking obesity and its related metabolic disorders. In the present study we use LPS to treat C2C12 skeletal muscle cells and observe expression of several inflammatory makers. Our results show that expression of inflammatory cytokine IL6 mRNA is strongly induced in the LPS treated C2C12 cells compared to the control cells. Surprisingly expression of the upper controller of inflammation TLR2 mRNA in the LPS treated C2C12 cells is similar as that in the control cells. Consistent with this expression mRNA level of TLR4 another upper regulator of inflammation is not differed between the two group cells. Taken together our current data suggests that the LPS induced expression of IL6 mRNA in C2C12 cells is not depend on the regulation of neither TLR2 nor TLR4. Keywords Lipopolysaccharide myotubes inflammation. 1. Introduction Raising of metabolic disorders such as insulin resistance type 2 diabetes liver hepatitis and heart diseases is a crucial health issue in the world 1 . Interestingly these metabolic dysfunctions are shown to be closely related with overweight and obesity which is usually induced by chronic high calorie intake 2 3 . Several studies have indicated that obesity related chronic inflammatory responses lead to many metabolic diseases. Inflammation is characterized by increased levels of inflammatory cytokines such as interleukin 6 IL6 and tumor necrosis factor alpha TNF α which in turn induced glucose and lipid metabolic dysfunctions. These changes are linking to the aforementioned disorders 4 5 . Lipopolysaccharide LPS the intermediate .
