Nghiên cứu này nhằm mục tiêu phân lập vi khuẩn có khả năng tổng hợp enzyme agarase từ nước biển và xác định hoạt tính của enzyme. Từ 8 mẫu nước biển thu thập tại các địa điểm khác nhau ở tỉnh Bà Rịa – Vũng Tàu. Mời các bạn tham khảo! | 50 Trường Đại học Nông Lâm TP. Hồ Chí Minh Isolation of agarase-producing bacteria from seawater and examination of the enzyme activity Lien T. H. Nguyen Phong V. Nguyen amp Thanh T. L. Bien Department of Biotechnology Nong Lam University Ho Chi Minh City Vietnam ARTICLE INFO ABSTRACT Research Paper This study aimed to isolate agarase-producing bacteria from seawater and then determine activity of the agarase. Eight Received September 20 2019 coastal surface seawater samples were collected from Ba Ria - Revised December 17 2019 Vung Tau province. Twenty-one bacterial strains that are ca- Accepted January 16 2020 pable of liquefying agar were isolated. These isolates produced disintegration zones around their colonies on agar plates with Keywords diameters ranging from to cm after an incubation period of 2 days at room temperature. Five bacterial strains Agarase M1 M5 M7 M62B and M71 that produced large halos on Agarolytic bacteria plates were identified belonging to Vibrio genus with identity Reducing sugar gt 96 . The crude enzyme activities of these strains ranged Seaweed from to U mL in reaction with agarose as substrate. Seawater Among isolated strains the strain M71 showed the highest agarase activity and was used to examine the degradation of Corresponding author seaweed. The hydrolysis of dried Gracilaria seaweed by the crude enzyme of M71 at concentration of 5 v v released 915 µM mL reducing sugar after a 24-h incubation period at 40o C. Bien Thi Lan Thanh Email bienthilanthanh@ Cited as Nguyen L. T. H. Nguyen P. V. amp Bien T. T. L. 2020 . Isolation of agarase-producing bacteria from seawater and examination of the enzyme activity. The Journal of Agriculture and Development 19 2 50-58. Tạp chí Nông nghiệp và Phát triển 19 2 Trường Đại học Nông Lâm TP. Hồ Chí Minh 51 Phân lập vi khuẩn tổng hợp enzyme agarase từ nước biển và xác định hoạt tính của enzyme Nguyễn Thị Hồng Liên Nguyễn Vũ Phong amp Biện Thị .