The Ryanodine Receptor-1 gene (RYR-1) is located in the 6p11 → q21 region on the No. 6 chromosome of pigs. RYR-1 is being investigated in several studies as a potentially important gene that is an indicator of pork quality. In this article, PCR-RFLP was used to analyze RYR-1 polymorphism in 19 Mong Cai pigs, 2 Yorkshire pigs and their 64 F1 generation pigs. | JOURNAL OF SCIENCE OF HNUE Chemical and Biological Sci. 2012 Vol. 57 No. 8 pp. 111-118 This paper is available online at http ANALYZING RYR-1 GENOTYPE POLYMORPHISM IN MONG CAI STOCK PIGS AND THEIR F1 GENERATION AND DESINGING A KIT FOR DETERMINING THIS GENOTYPE IN PIGS Trieu Anh Trung1 Nguyen Van Anh2 Tran Thi Quynh Anh3 and Nguyen Van Cuong3 1 Faculty of Biology Hanoi National University of Education 2 Faculty of Biology Thai Nguyen University of Education 3 Vietnam Academy of Science and Technology Abstract. The Ryanodine Receptor-1 gene RYR-1 is located in the 6p11 q21 region on the No. 6 chromosome of pigs. RYR-1 is being investigated in several studies as a potentially important gene that is an indicator of pork quality. In this article PCR-RFLP was used to analyze RYR-1 polymorphism in 19 Mong Cai pigs 2 Yorkshire pigs and their 64 F1 generation pigs. The DNA fragment of 656 bp of RYR-1 was amplified using PCR that employs specific primers and is then digested by Hin6I. The results showed that Mong Cai pigs had only the homozygote NN 100 at the RYR-1 locus while imported Yorkshire pigs had both the homozygote NN and the heterozygote Nn genotypes. Of their F1 generation five out of 64 pigs were of the Nn genotype and the others were of the NN genotype. We designed and developed a Master Mix kit set that can be used to identify RYR-1 in pigs DNA genome. This kit can be used by breeding farms and for breeding programs. The kit can be used to quickly determine the RYR-1 genotype of pigs. The kit is very accurate and the measurements obtained are very reliable. Keywords Ryanodine Receptor-1 gene RYR-1 porcine stress syndrome PSS malignant hyperthermia disease MH PCR-RFLP. 1. Introduction In Vietnam conventional pig breeding methods are the norm in agricultural production. There exist various local breeds of pigs and they are well adapted to the local Received June 28 2012. Accepted July 25 2012. Biology Subject Classification C400. Contact Trieu .
