Tối ưu hóa các điều kiện sản xuất vaccine bất hoạt phòng bệnh gan thận mủ cho cá tra

Nghiên cứu này được thực hiện nhằm tối ưu hóa các điều kiện sản xuất vaccine sốc nhiệt bất hoạt phòng bệnh gan thận mủ trên cá tra. Chủng vi khuẩn dùng làm vaccine được phân lập và chọn lọc từ mẫu cá tra bệnh gan thận mủ thu tại các tỉnh Tiền Giang, Đồng Tháp và An Giang. Với phương pháp sốc nhiệt và kiểm tra protein sốc nhiệt bằng phản ứng SDS-PAGE và Western Blot cho thấy ở điều kiện gây sốc 41o C trong 30 phút E. ictaluri sinh lượng heat shock nhiều hơn so với các điều kiện thử nghiệm khác. | VIỆN NGHIÊN CỨU NUÔI TRỒNG THỦY SẢN 2 STUDY OF TRANSMISSION OF INFECTIOU HYPERDERMAL AND HEMATOPIETIC NECROSIS VIRUS IHHNV IN BLACK TIGER SHRIMP PENEUS MONODON Cao Thanh Trung1 Pham Cong Nguyen1 Nguyen Van Hao2 ABSTRACT The infectious hypodermal and haematopoietic necrosis virus IHHNV also called Penaeus styl- irostris densovirus PstDNV has been identified high mortality Penaeus stylirostris in the Ameri- cas. It has been reported to cause Runt deformity syndrome RDS in Penaeus vannamei which is a chronic non-lethal disease. At present there is no established experimental model of infection in Penaeus monodon under laboratory conditions. Transmission of IHHNV have been experimented by the horizontal and vertical routes. Horizontal transmission were performed with 3 trials which are fed with the infected shrimps co-habitant combine with feeding and cohabitant. The vertical transmission from infected females to offspring have been demonstrated. The IHHNV infection was monitored by PCR assay. Result of experiments were showed that IHHNV vertical transmission from infected females were clearly proved. All life stages that produced from infected females were presented the IHHNV by PCR assay. In horizontal transmission the free IHHNV of P. monodon in all trials that were positive with the IHHNV at 14 days and 28 days after the infection. In the trial of cohabiting IHHNV infected P. mondon was and feeding with IHHNV infected P. mondon was and combine feeding and cohabitan was and . In infection with difference of species trials. The percent of infected IHHNV of P. monodon of cohabitant trial was and after infected at 14 days and 28 days feeding trial was and and the combine feeding and cohabitant trial was and . Experimental infection could be used to study the P. monodon pathogen further in order to prevent and control of virus infected in shrimps. Keywords IHHNV Penaeus monodon transmission Người phản biện TS.