Bài viết phân tích sự tăng sinh tế bào (MTT), chúng tôi đã chỉ ra rằng curcumin có khả năng ức chế rõ rệt sự tăng sinh của tế bào ung thư dạ dày MKN45. Các phân tích bằng Flow cytometry cho thấy rằng, curcumin đã làm tăng tỷ lệ % tế bào apoptosis từ 27% đến 56% ở các nồng độ từ 10µM - 20µM và làm xuất hiệu kiểu nhân điển hình của tế bào apoptosis. Xa hơn nữa, nghiên cứu này đã chỉ ra rằng, curcumin đã điều hòa giảm sự biểu hiện của protein aldehyde dehydrogenase trong tế bào ung thư dạ dày MKN45. | VNU Journal of Science Natural Sciences and Technology Vol. 36 No. 3 2020 80-87 Original Article Curcumin Inhibits Cell Proliferation Stimulates Apoptosis and Down-Regulates Aldehyde Dehydrogenase Expresion in Gastric cancer Cell line MKN45 Nguyen Phu Hung1 Le Thi Thanh Huong1 Nguyen Trung Thanh2 1 Faculty of Biotechnology Thai Nguyen University of Sciences Thai Nguyen University Z115 street Tan Thinh ward Thai Nguyen city Thai Nguyen province Vietnam 2 Faculty of Biology VNU University of Science 334 Nguyen Trai Hanoi Vietnam Received 10 May 2020 Revised 28 August 2020 Accepted 30 August 2020 Abstract According to estimates by the World Health Organization WHO in 2018 there were over one million new stomach cancer patients. Vietnam ranks the tenth among the countries with the highest rates of stomach cancer in the world with the rate of cases per 100 000 populations. Research on compounds or drugs that can inhibit cancer cell growth but are less toxic to the body is necessary. In this study using MTT assays we have shown that curcumin has ability to inhibit proliferation of stomach cancer cells MKN45. Flow cytometry analysis showed that curcumin increased the percentage of apoptosis cells by 27 - 56 at concentrations of 10 µM - 20 µM and resulted in a typical nuclear morphology of apoptosis. Further this study showed that curcumin significantly reduced the expression of aldehyde dehydrogenase protein in MKN45 gastric cancer cells. This finding shows that curcumin is a potential therapeutic candidate for gastric cancer cell treatment. Keywords curcumin gastric cancer stem cell cancer stem cell marker aldehyde dehydrogenase. ____ Corresponding author. Email address hungnguyenphu@ https 2588-1140 80 . Hung et al. VNU Journal of Science Natural Sciences and Technology Vol. 36 No. 3 2020 80-87 81 Curcumin ức chế sự tăng sinh cảm ứng apoptosis và làm giảm sự biểu hiện của aldehyde dehydrogenase ở tế bào ung thư dạ dày
