In the present study, 3 mongrel dogs were taken for semen collection. Total of 18 ejaculates were collected, 6 from each dog by digital manipulation. All the collected samples were randomly divided into three Groups viz. Group I (Non-extended semen), Group II (Semen extended with egg yolk tris citrate fructose extender) and Group III (Sperm pellet extended with egg yolk tris citrate fructose extender) and kept at refrigeration temperature (4oC). In Group III semen is centrifuged at 720 x g for 5 minutes by centrifuge machine Tarsons Spinwin MC05-R for making of sperm pellet. |
