Smilax glabra Roxb. is a medicinal plant of Vietnam to treat several diseases such as: Piercing pain in the bones, detoxicating, scabies, nephritis, cystitis, syphilis, . From ethanol extracts of Smilax glabra Roxb. cultivating in Thai Nguyen, 5 compounds were isolated and structurally elucidated in the first time by various spectral methods, such as IR, MS, 1H-NMR, 13C-NMR, DEPT, HSQC, HMBC. | JOURNAL OF SCIENCE OF HNUE Natural Sci. 2010 Vol. 55 No. 6 pp. 62-70 STUDY ON THE CHEMICAL CONSTITUENTS IN ROOTS OF Smilax glabra Roxb. CULTIVATED IN THAI NGUYEN PROVINCE Pham Thi Hong Minh Nguyen Quyet Tien Institute of Chemistry VAST Tran Thi Thanh Hang Thai Nguyen University of Education Pham Huu Dien Hanoi National University of Education E-mail dienhp@ Abstract. From the ethanol extract of the Smilax glabra growing in Thai Nguyen province five compounds such as stigmasta-5 22-diene-3β-ol 1 3β-sitosterol-glucopyranoside 2 4 5 7-trihydroxyflavanone-3-O-α-L- rhamnopyranoside engeletin 3 3 5 5 7-tetrahydroxyflavanone-3-O-α-L- rhamnopyranoside smitilbin 4 and 3 4 5 7-tetrahydroxyflavanone-3-O- α-L-rhamnopyranoside astilbin 5 were isolated. Their chemical struc- tures were elucidated by IR ESI-MS 1D and 2D NMR experiments. Keywords Smilax glabra Roxb. isolation steroits engeletin smitilbin astilbin 1. Introduction Smilax glabra Roxb. plant belongs to the Smilacaceae family wildly growing in several Provinces of Vietnam such as Lang Son Quang Ninh Tien Yen Thai Nguyen Dai Tu Bac Giang Vinh Phuc Hai Duong Ha Tay Hoa Binh Ninh Binh Nghe An Hue Da Nang Quang Nam Kon Tum Lam ong Khanh Hoa Ninh Thuan. In the world Smilax glabra Roxb. plant is discovered in India Myanma China Laos Cambodia and Thailand. Smilax glabra Roxb. is medicinal plant of Vietnam to treat several diseases such as piercing pain in the bones detoxication scabies nephritis cystitis syphilis . 1 2 . Some compounds were found from Smilax glabra Roxb. roots such as dihydrokaempferol 3-O-α-L-rhamnopyranoside and astilbin 3 . 62 Study on the chemical constituents in roots of Smilax glabra Roxb. 2. Content . Materials and methods . General experimental procedures Melting points were determined by using an Electrothermal IA-9200 appara- tus. IR spectra was obtained by an IMPACT 410 type spectrometer in KBr disks. EI-MS was obtained by using a Hewlett Packard 5989 B MS spectrometer. ESI-MS was
